Target Search

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AT4G27890.1 | Symbols:  | nuclear movement family protein | chr4:13886039-13888282 FORWARD
Target region: GAGGAGGCGAGACCCTCGATG

Hybridization energy: -38.09kcal/mol (75.32 %)

Target gene 5'->3'/22-42	
GAGGAGGCGAGACCCTCGATG
amiRNA (rev. complement)   	
GAGGGGGAGAGACCCTCGATA
	
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AT1G55470.1 | Symbols:  | transposable element gene | chr1:20713143-20714977 REVERSE
Target region: GAGGGGGAGATACTTTCGATA

Possible candidate. Mismatch at position 10 and/or 11

Hybridization energy: -37.83kcal/mol (74.81 %)

Target gene 5'->3'/426-446	
GAGGGGGAGATACTTTCGATA
amiRNA (rev. complement)   	
GAGGGGGAGAGACCCTCGATA
	
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AT5G14720.1 | Symbols:  | protein kinase family protein | chr5:4747863-4753595 REVERSE
Target region: GAGGAGGAGAGAAACTCGATA

Possible candidate. More than one mismatch at positions 2-12

Hybridization energy: -34.32kcal/mol (67.87 %)

Target gene 5'->3'/26-46	
GAGGAGGAGAGAAACTCGATA
amiRNA (rev. complement)   	
GAGGGGGAGAGACCCTCGATA
	
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AT2G18193.1 | Symbols:  | AAA-type ATPase family protein | chr2:7924587-7926359 REVERSE
Target region: GAGGCGAAGAGATCCTCGATA

Possible candidate. Low binding affinity: Only 61.12% of optimal dG

Hybridization energy: -30.91kcal/mol (61.12 %)

Target gene 5'->3'/422-442	
GAGGCGAAGAGATCCTCGATA
amiRNA (rev. complement)   	
GAGGGGGAGAGACCCTCGATA
	
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